Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.
نویسندگان
چکیده
Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression.
منابع مشابه
Complete Genome Sequence of a thermotolerant sporogenic lactic acid bacterium, Bacillus coagulans strain 36D1
Bacillus coagulans is a ubiquitous soil bacterium that grows at 50-55 °C and pH 5.0 and ferments various sugars that constitute plant biomass to L (+)-lactic acid. The ability of this sporogenic lactic acid bacterium to grow at 50-55 °C and pH 5.0 makes this organism an attractive microbial biocatalyst for production of optically pure lactic acid at industrial scale not only from glucose derive...
متن کاملContributory roles of two l-lactate dehydrogenases for l-lactic acid production in thermotolerant Bacillus coagulans
Thermotolerant Bacillus coagulans is considered to be a more promising producer for bio-chemicals, due to its capacity to withstand harsh conditions. Two L-lactate dehydrogenase (LDH) encoding genes (ldhL1 and ldhL2) and one D-LDH encoding gene (ldhD) were annotated from the B. coagulans DSM1 genome. Transcriptional analysis revealed that the expression of ldhL2 was undetectable while the ldhL1...
متن کاملBacillus subtilis as a Host for Recombinant Hemagglutinin Production of the Influenza A (H5N1) Virus
Abstract Background and Aims: Influenza A(H5N1) viruses circulating in animals might evolve and acquire the ability to spread from human to human and thus start a pandemic. Hemagglutinin (HA) has been shown to play a major role in binding of influenza virus to its target cell and the main neutralizing antibody responses elicit against this region. Recent studies have shown that...
متن کاملBacillus coagulans MA-13: a promising thermophilic and cellulolytic strain for the production of lactic acid from lignocellulosic hydrolysate
BACKGROUND The transition from a petroleum-based economy towards more sustainable bioprocesses for the production of fuels and chemicals (circular economy) is necessary to alleviate the impact of anthropic activities on the global ecosystem. Lignocellulosic biomass-derived sugars are suitable alternative feedstocks that can be fermented or biochemically converted to value-added products. An exa...
متن کاملMolecular Typing and Probiotic Attributes of a New Strain of Bacillus coagulans – Unique IS-2: a Potential Biotherapeutic Agent
The genus Bacillus has been in use in the biotechnology industry for a very long time with a number of new cultures exhibiting a variety of benefits to humans. The present study focuses on phenotypic and genotypic characterization of a probiotic lactic acid bacteria, Bacillus coagulans (IS-2 a Unique-Biotech strain) isolated from human feces and contaminated soils. Probiotics are live microbes ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 76 12 شماره
صفحات -
تاریخ انتشار 2010